P-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture
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Abstract:
Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged in LN2 by acupuncture needle. Medium sized preantral follicles of fresh control and vitrified-warmed ovaries were mechanically isolated and cultured in fibrin-alginate matrix for 12 days. Finally, survival and growth rate and also quantitative oocyte maturation genes (Gdf9 and Bmp15) expression of follicles were evaluated on 1st and 12th culture days Results Although higher follicle survival rate was shown in control group rather than vitrification one, follicle diameter was being increased until the last culture day in both groups. Quantitative oocyte maturation genes expression evaluation did not reveal any significant difference between control and vitrification groups on first and last days of culture, but they were significantly decreased on 12th culture day compared to the first in both groups Conclusion According to the observed similarities in both groups, it seems that fibrin-alginate could be an appropriate matrix for mouse preantral follicle development and in spite of decreased follicle survival rate in vitrification group, applied vitrification method did not affect developmental competence of ovarian follicles
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Journal title
volume 9 issue 2
pages 78- 78
publication date 2015-09-01
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